Effect of Shenmai injection on immune function in rats after scald

Objective To investigate the regulatory effect of Shenmai injection on immunity function in rats with severe scald injury.Methods Fifty-six healthy SD rats were randomly divided into three groups:control group(n=8),model group(n=24)and Shenmai group(n=24).Rats models with 30% TBSAⅢ degree scald burn were established in model group and Shenmai group,and were intraperitoneally injected normal saline and Shenmai injection one hour after the establishment,respectively,twice a day.And control group was intraperitoneally injected normal saline,twice a day.By day 3,6and 9after scald,the counts of CD3+,CD4+and CD8+in peripheral blood were measured by monoclonal antibody APAAP immunocytochemical method,and the CD4+/CD8+ratio was calculated.The levels of IgA,IgG and IgM were measured by immune turbidity assay.Results The counts of CD3+((56.5±3.4)%)and CD4+((39.1±4.1)%)were significantly lower and the count of CD8+((29.7±2.6)%)was significantly higher in model group than those in control group(CD3+:(69.3±4.1)%,CD4+:(49.1±3.7)%,CD8+:(21.6±3.4)%)(P0.05).The ratio of CD4+/CD8+was lower in model group(1.32(1.21,1.47))than that in control group(2.20(1.96,2.51))(P0.05).The levels of IgA((199.1±18.6)mg/L),IgG((181.6±20.3)mg/L)and IgM((200.4±22.6)mg/L)were significantly lower in model group than those in control group((230.1±22.2),(187.3±23.7),(229.5±24.5)mg/L)(P0.05).By day 9after scald in Shenmai group,the counts of CD3+((68.2±3.5)%)and CD4+((48.4±4.20)%),CD4+/CD8+ratio(2.37±1.08),and the levels of IgA((229.4±19.8)mg/L),IgG((193.5±19.8)mg/L)and IgM((230.4±21.7)mg/L)were significantly higher than those in model group,and the count of CD8+((20.2±3.9)%)was significantly lower in Shenmai group than that in model group(P0.05).There were no significant differences in the above indexes between Shenmai group and control group(P0.05).Conclusion Shenmai injection could efficiently enhance the immune function in rats with severe scald.