TaqMan PCR-based methods for rapid detection of Mycobacterium avium subsp. paratuberculosis in deer blood

For detection Mycobacterium avium subsp.paratuberculosis(MAP) in deer blood,a TaqMan PCR assay was developed.It targets and amplifies sequences from the f57 insertion element which is specific for this bacterium,the assay was tested against MAP and other mycobacterium strains.It is capable of detecting single cell.A total number of 549 serum samples collected from Changchun were tested by TaqMan PCR,the positive detection rates of these methods were 18.4%.The results showed that TaqMan PCR assay was rapid and accuracy for detection of MAP from infected deer products.