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Construction of Pichia pastoris yeast strain with high-efficient expression of HSA-OPG

Liu Jun

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Abstract

Objective:To fuse the gene of osteoprotegerin(OPG) with that of human serum albumin(HSA) in order to construct a Pichia pastoris yeast strain with high-efficient expression of HSA-OPG.Methods:The gene of OPG was amplified by RT-PCR and the plasmid of pHILD2-HSA-OPG was constructed.HSA-OPG fusion protein was detected with ELISA kit.Results:The expression plasmid of pHIL-D2-HSA-OPG was proved to be correct after plamid identification and sequencing,and rhHSA-OPG was relatively efficiently expressed in Pichia pastoris.Conclusion:Pichi pastoris yeast strain with high-efficient expression of HSA-OPG is successfully constructed,providing a basis for further study of its bioactivity.

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What this paper is about

Objective:To fuse the gene of osteoprotegerin(OPG) with that of human serum albumin(HSA) in order to construct a Pichia pastoris yeast strain with high-efficient expression of HSA-OPG.Methods:The gene of OPG was amplified by RT-PCR and the plasmid of pHILD2-HSA-OPG was constructed.HSA-OPG fusion protein was detected with ELISA kit.Results:The expression plasmid of pHIL-D2-HSA-OPG was proved to be correct after plamid identification and sequencing,and rhHSA-OPG was relatively efficiently expressed in Pichia pastoris.Conclusion:Pichi pastoris yeast strain with high-efficient expression of HSA-OPG is successfully constructed,providing a basis for further study of its bioactivity.

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Available abstract

Objective:To fuse the gene of osteoprotegerin(OPG) with that of human serum albumin(HSA) in order to construct a Pichia pastoris yeast strain with high-efficient expression of HSA-OPG.Methods:The gene of OPG was amplified by RT-PCR and the plasmid of pHILD2-HSA-OPG was constructed.HSA-OPG fusion protein was detected with ELISA kit.Results:The expression plasmid of pHIL-D2-HSA-OPG was proved to be correct after plamid identification and sequencing,and rhHSA-OPG was relatively efficiently expressed in Pichia pastoris.Conclusion:Pichi pastoris yeast strain with high-efficient expression of HSA-OPG is successfully constructed,providing a basis for further study of its bioactivity.

Key concepts: Pichia pastoris, Pichia, Human serum albumin, Yeast, Fusion protein, Plasmid, Osteoprotegerin, Recombinant DNA

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