Influence of histamine on interleukin-2 production and proliferation of T lymphocytes in vitro

Objective:To investigate the influence of histamine on intedeukin(IL-2) production and proliferation of CD4+ T lymphocytes and CD8+ T lymphocytes.Methods:Density gradient centrifugalization and absorption technique were respecitively applied to detach peripheral blood mononuclear cells(PBMC) and peripheral blood lymphocytes(PBLC). CD4+ and CD8+ T lymphocytes divided with anti-CD4+ and anti-CD8+ antibody were cultured in vitro. IL-2 levels in supernatant were quantified by enzyme-linked immunosobent assay (ELBA), and proliferation index were measured by MTT methods. Results:IL-2 levels in supernatant and proliferation rate of CD4+ and CD8+ T lymphocytes treated with histamine were significantly lower than those of their corresponding un-stimulated controls(P0.05). IL-2 production and proliferation rate of CD4+ (CD8+) T lymphocytes co-cultured with histamine and cimetidine were higher than those of the controls cultured with histamine alone( P 0.05). CD4+ T lymphocyte produced much more IL-2 than CDS* . Conclusion: Histamine inhibit T lymphocytes proliferation and IL-2 production,and the inhibitory effect can be stocked by cimetidine.CDS* T lymphocytes also secret IL-2,but less than CD4+ T cell.