Effects of Nitrogen Forms on Free Amino Acid Content of Organs above Ground and Grain Protein of Yumai 34

The study aimed to analyze Effects of nitrogen forms on free amino acid content of organs above ground and grain protein of Yumai 34.The results showed that the free amino acid content of different leaves,stem,sheath and grain reached the maximum at anthesis,and with the highest in ear axis and husks at 10 days after anthesis.The content in different leaves all declined as the order as the flag leaf﹥2 nd leaf﹥3 rd leaf﹥4 th leaf.Among the three kinds of nitrogen forms,NH2——N treatment had the highest content in organs before 30 days after anthesis.NH4+-N treatment or NO3——N treatment had higher contents in the 3 rd leaf,the 4 th leaf,ear axis and husks at anthesis.Yumai 34 had the highest content of globulin with NO3——N treatment,the highest content of gliadin and glutenin with NH4+-N treatment,and the highest content of albumin,ratio of glutenins/gliadins and protein content with NH2——N treatment.Applying urea fertilizer could increase the free amino acid content of different organs above the ground in the prior and middle stage of grain filling and that could advance the synthesis of grain protein and increase the content of grain protein in the late stage of grain filling.and urea was the first choice of nitrogen forms in the quality cultivation of Yumai 34.