Isolation and Identification of Antagonistic Bacteria against Fusarium Wilt Tomato

[Objective]Fusarium wilt is one of the main diseases of tomato with its occurrence increasingly serious in recent years and causes severe damage to yield and quality of tomato.[Method] Methods,such as plate screening,phylogenetic analysis of 16S rRNA,flat confrontation culture and fermented broth antagonistic experiments were used.[Result]64 strains of bacteria have been isolated from the soil planted tomato continuously for many years in Xinjiang,7 isolates showed certain antagonism against Fusarium wilt pathogen(Fusarium oxysporum(Sch1) f.sp lycopersici) in the preliminary test,and the isolates B -5 and LYT - 5 could inhibit F.oxysporum significandy in revaluation,which were identified respectively as similar species of Bacillus amyloliquefaciens and Bacillus velezensis by phylogenetic analysis of 16S rRNA. Confrontation test showed that B - 5 and LYT - 5 inhibited the growth of F.oxysporum with a wide inhibiting zone.Fermented broth antagonistic experiments showed that B - 5 and LYT - 5 could resist F.oxysporum obviously with its inhibition rate of 100%for 5 days and 98%for another for 5 days.[Conclusion]The result shows that the two isolates with significant antagonistic effect to F.oxysporum could provide potential solution for biological control of Fusarium wilt of tomato.