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2013•Chinese Archives of Otolaryngology-head and Neck SurgeryRequires access

BCL-2 protein highly expressing in nasopharyngeal carcinoma cells sensitize to cisplatin-induced apoptosis

Miao Beiping

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Abstract

Objective:To explore a new target of cisplatin-Induced apoptosis.Method:The expressions of BCL-2 protein in three types of nasopharyngeal carcinoma cells CNE1、C666-1 and SUNE1 were detected by Western blot;after treated with cisplatin,the survival rates of the three cells were evaluated with MTT assay,and the apoptosis rates were detected by flow cytometr.Result:BCL-2 protein was highly expressing in CNE1、C666-1,while there was nearly no in SUNE1(P0.05).The survival rates of CNE1 and C666-1 were significantly increased and the apoptosis rates were decreased obviously after treated with cisplatin(P0.05),while the effect of the cisplatin was poor on SUNE1.Conclusion:The expression of BCL-2 protein was different in different type of NPC cells;the NPC BCL-2 protein highly experssion were sensitived to cisplatin-induced apoptosis.

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Objective:To explore a new target of cisplatin-Induced apoptosis.Method:The expressions of BCL-2 protein in three types of nasopharyngeal carcinoma cells CNE1、C666-1 and SUNE1 were detected by Western blot;after treated with cisplatin,the survival rates of the three cells were evaluated with MTT assay,and the apoptosis rates were detected by flow cytometr.Result:BCL-2 protein was highly expressing in CNE1、C666-1,while there was nearly no in SUNE1(P0.05).The survival rates of CNE1 and C666-1 were significantly increased and the apoptosis rates were decreased obviously after treated with cisplatin(P0.05),while the effect of the cisplatin was poor on SUNE1.Conclusion:The expression of BCL-2 protein was different in different type of NPC cells;the NPC BCL-2 protein highly experssion were sensitived to cisplatin-induced apoptosis.

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Available abstract

Objective:To explore a new target of cisplatin-Induced apoptosis.Method:The expressions of BCL-2 protein in three types of nasopharyngeal carcinoma cells CNE1、C666-1 and SUNE1 were detected by Western blot;after treated with cisplatin,the survival rates of the three cells were evaluated with MTT assay,and the apoptosis rates were detected by flow cytometr.Result:BCL-2 protein was highly expressing in CNE1、C666-1,while there was nearly no in SUNE1(P0.05).The survival rates of CNE1 and C666-1 were significantly increased and the apoptosis rates were decreased obviously after treated with cisplatin(P0.05),while the effect of the cisplatin was poor on SUNE1.Conclusion:The expression of BCL-2 protein was different in different type of NPC cells;the NPC BCL-2 protein highly experssion were sensitived to cisplatin-induced apoptosis.

Key concepts: Cisplatin, Nasopharyngeal carcinoma, Apoptosis, Western blot, Flow cytometry, BAX Protein, MTT assay, Cancer research

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BCL-2 protein highly expressing in nasopharyngeal carcinoma cells sensitize to cisplatin-induced apoptosis — Research Paper | ScholarLens