Study on histology impact of chickens' bursa of fabricius after infection with infectious bursal disease virus by mark form

To establish the animal model of infectious bursal disease and provide the data base and basic theories of this diseases,5 weeks old SPF chickens were infected by a IBDV strain BC6/85,histolpathological and Immunohistochemistrical methods were used to study the change of the pathological lesions and IBDV immunoreactive positive cells in bursa of fabricius from 2 to 432 h after infection.Results showed that IBDV and pathological lesion could be observed in the chamber and follicles of the bursa of fabricius in infected chickens and the IBDV detected after lesions appearing.Both of the pathological lesion and IBDV immunoreactive positive cells in bursa of fabricius increased at the beginning and then became different because of the positive cells disapeared with the disapearances of the bursal follicles.IBDV and bursal follicles still can be detected in the busal of infected chickens in the later period of infection.The study detail recorded the whole phases of the disease of the chickens infacted IBDV strain BC6/85 and installed the preliminary animal model.Then the form of marking was used to analyze the histological bursa lesions together with the density of IBDV immunoreactive positive cells,and searched mechanism of pathogenic.