Effects of Nuclear Factor-kappa B Decoy Oligonucleotide on the Apoptosis of HepG2 Cells

Objective To investigate the effect of NF-κB decoy oligodeoxynucleotides(ODNs)on the activity of NF-κB and its mechanism in apoptosis of liver cancer cells HepG2.Methods The NF-κB decoy ODNs was designed,FITC-labelled NF-κB decoy ODNs was transfected into HepG2,the nuclear localization was observed with confocal microscope,the growth curve was established;the activity change of NF-κB was detected with electrophoretic mobility shift assays(EMSA)pre-and post-transfection;the effect of low activity NF-κB on the cell proliferation was evaluated with flow cytometry and TUNEL,the expression change of Bcl-2 and Fas induced with low activity NF-κB were measured with Western blot.Results The decoy ODNs was located in the nuclei of HepG2 cells,the activity of NF-κB was significantly decreased,the growth of HepG2 was significantly inhibited,the apoptosis of liver cancer cells HepG2 was enhanced;the low activity of NF-κB upregulated the expression of Fas and downregulated the expression of Bcl-2.Conclusion The NF-κB decoy ODNs could increase the apoptosis of liver cancer cells HepG2 and its mechanism may be related to the downregulation of NF-κB activity,upregulation of Fas expression and downregulation of Bcl-2 expression.