2007•Yumi kexueRequires access
Study on Induction Embryogenic Callus and Plant Regeneration in Maize Inbred Lines
Piwu Wang
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Abstract
In experiment, factors influencing the callus induction of immature embryos in maize inbred lines 7922, H99, P2, Mo17, Ji846, Ji63, etc. Such as different media, different concentrations of phytohormone, different carbon sources and their concentrations were studied. The results showed that NB was optimal media of callus induction on immature embryos in different maize inbred lines, concentration of 2,4-D 2 mg/L could obviously enhance inducing frequency, concentration of sucrose 20 g/L and 20 g/L glucose or sucrose 30 g/L and 10 g/L glucose was the better conditions of callus induction, but could not be used in long period. More over, this paper discussed the effects of different hormone on regeneration of maize callus.