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2006•Zhongguo jihua mianyiRequires access

Vero/Slam Cell Application in Isolation of Measles Virus

Guo Xue

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Abstract

Objective To study the sensitivity of Vero/Slam cell to measles virus(MV) and characteristics of MV in Vero/Slam cell.Methods Histiocyte culture.The samples were inoculated in the single layer Vero/Slam cells,up to 3 blind passages,and observed the MV CPE.The MV from these samples were isolated using Vero/Slam cell line,450 nucleotides of COOH-terminal of nudeoprotein genes were amplified and sequence analyzed by RT-PCR and sequence analysis software.Results Vero/Slam cell line is highly sensitive to measles virus.The cytopathic effect(PE) characteristics of measles virus in Vero/Slam cell was cell fusion.The isolated wild-type measles virus is H_1,the same geno-type as Chinese native prevailing measles virus.Conclusion Vero/Slam cell line is sensitive,it can be used in isolation of measles virus.

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What this paper is about

Objective To study the sensitivity of Vero/Slam cell to measles virus(MV) and characteristics of MV in Vero/Slam cell.Methods Histiocyte culture.The samples were inoculated in the single layer Vero/Slam cells,up to 3 blind passages,and observed the MV CPE.The MV from these samples were isolated using Vero/Slam cell line,450 nucleotides of COOH-terminal of nudeoprotein genes were amplified and sequence analyzed by RT-PCR and sequence analysis software.Results Vero/Slam cell line is highly sensitive to measles virus.The cytopathic effect(PE) characteristics of measles virus in Vero/Slam cell was cell fusion.The isolated wild-type measles virus is H_1,the same geno-type as Chinese native prevailing measles virus.Conclusion Vero/Slam cell line is sensitive,it can be used in isolation of measles virus.

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Available abstract

Objective To study the sensitivity of Vero/Slam cell to measles virus(MV) and characteristics of MV in Vero/Slam cell.Methods Histiocyte culture.The samples were inoculated in the single layer Vero/Slam cells,up to 3 blind passages,and observed the MV CPE.The MV from these samples were isolated using Vero/Slam cell line,450 nucleotides of COOH-terminal of nudeoprotein genes were amplified and sequence analyzed by RT-PCR and sequence analysis software.Results Vero/Slam cell line is highly sensitive to measles virus.The cytopathic effect(PE) characteristics of measles virus in Vero/Slam cell was cell fusion.The isolated wild-type measles virus is H_1,the same geno-type as Chinese native prevailing measles virus.Conclusion Vero/Slam cell line is sensitive,it can be used in isolation of measles virus.

Key concepts: Vero cell, Measles virus, Virology, Virus, Canine distemper, Paramyxoviridae, Morbillivirus, Cytopathic effect

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