Analysis on the Soluble Protein Expression Profile in Diapause Eggs of Silkworm,Bombyx mori,Treated With HCl

To find the proteins associated with diapause termination for silkworm eggs with HCl treatment will be beneficial to improve and enhance the artificial incubation techniques of silkworm eggs.Using the dipause eggs stored at 2.5 ℃ for 45 days as materials,the soluble protein expression profile of diapause eggs was investigated by two-dimensional electrophoresis(2-DE) and image analysis software.For the group untreated with HCl,523 protein spots were detected in the 2-DE image,including 41 unique protein spots.Meanwhile,for the group treated with HCl,526 protein spots were identified,including 44 unique protein spots.For the 2 groups,the number of protein spots matched were 482 pairs and the ratio of match was 91.897%.By contrasting analysis,we found that there existed some unique protein spots and some protein spots which protein content was changed significantly in the group treated with HCl,suggested that these proteins maybe asociated with diapause termination.