The Research on the Clone Construction about Hypocotyl and Cotyledon of Six Kinds of Brassica Oleracea

In this experiment,the paper studies the establishment of clone system and tissue culture about different organs of six varieties of Brassica oleracea,and succeeds in inducing the stalk tips,hypocotyls or cotyledons of Brassica oleracea L var.capitata L, Brassica oleracea var.bullata D.C,Brassica oleracea var.sabellica L, Brassica oleracea L var.germmifera Zenk,Brassica oleracea L var.caulorapa D.C and Brassica oleracea var.alboglabra Bailey to differentiate uncertain buds.The best medium to induce different organs of the six kinds of Brassica oleracea is MS+6-BA0.5~1mg/L;the ideal rooting medium for the uncertain buds is MS+IAA0.2~0.5mg/L;the best medium for transplanting the tube seeding is cinder.The growth condition of the tube plants is also favorable.