Study on Extraction of Polysaccharides from Orange Peel with Microwave
Yue Xian-tian
Abstract
Yue Xian-tian
Abstract
[Objective]The study was to provide the technical support for the comprehensive utilization of orange peel.[Method]The orange peel was crushed and dried.The polysaccharide in it was extracted with ethanol as solvent assisted by heating and ultrasonic.The content of polysaccharide in the extract was measured by phenol-sulfuric acid method.The effects of different extraction conditions on extraction rate of polysaccharides were analyzed.[Results] The results of single factor experiment showed that when the heating extraction time was 4 h,the extraction temperature was 80 ℃,the solid-liquid ratio was 1∶50,the microwave power was 500 W,the irradiation time was 5 min,the extracting times was 6 times,the ethanol concns.was 100% resp.the extraction rate of polysaccharide was the highest.The optimum extraction conditions was heating extraction time of 4 h,extraction temperature of 80 ℃,solid-liquid ratio of 1∶50,extraction times of 4 times,ethanol concns.of 80%,microwave power of 500 W,irradiation time of 5 min.Under this condition,the extraction rate of polysaccharide reached 15.23%.[Conclusion] This study identified the optimum extraction conditions of polysaccharide in orange peel.
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[Objective]The study was to provide the technical support for the comprehensive utilization of orange peel.[Method]The orange peel was crushed and dried.The polysaccharide in it was extracted with ethanol as solvent assisted by heating and ultrasonic.The content of polysaccharide in the extract was measured by phenol-sulfuric acid method.The effects of different extraction conditions on extraction rate of polysaccharides were analyzed.[Results] The results of single factor experiment showed that when the heating extraction time was 4 h,the extraction temperature was 80 ℃,the solid-liquid ratio was 1∶50,the microwave power was 500 W,the irradiation time was 5 min,the extracting times was 6 times,the ethanol concns.was 100% resp.the extraction rate of polysaccharide was the highest.The optimum extraction conditions was heating extraction time of 4 h,extraction temperature of 80 ℃,solid-liquid ratio of 1∶50,extraction times of 4 times,ethanol concns.of 80%,microwave power of 500 W,irradiation time of 5 min.Under this condition,the extraction rate of polysaccharide reached 15.23%.[Conclusion] This study identified the optimum extraction conditions of polysaccharide in orange peel.
Key concepts: Polysaccharide, Chemistry, Chromatography, Extraction (chemistry), Orange (colour), Solvent, Ethanol, Sulfuric acid