2008•He'nan nongye kexueRequires access
Application of 6×His-tag in the Expression of Antibacterial Peptide and its Effect on the Antibacterial Activity
Qiang Gong
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Abstract
For the convenience of expression and purification of antimicrobial peptides in vitro,the scorpion defensin gene(sd) and the gene fused with 6×His-tag(sd-His) were reconstructed and expressed in P.pastoris.The two peptides were purified and then the antibacterial activity,thermal and acid stability were detected in vitro.Surprisingly,the antibacterial activity of Sd-His had not been reduced but slightly enhanced.The results indicate that 6 × His-tag can be used for the fusion expression of antimicrobial peptide without affecting its activity.This study provides a simple and efficient method for the expression and purification of antimicrobial peptide.