Application of Gradient Centrifugation In Vitro Treatment of Sperm-binding Antibody

Objective:To compare the PureSperm technique alone and the PureSperm combined with swim-up method in processing the sperm-binding antibody positive sperm.Methods:The PureSperm technique alone and the PureSperm combined with swim-up method were used to process in vitro the sperm-binding antibody positive sperm from patients with male sterility.Results:The titer of sperm-binding antibodies was significantly declined after treatment by both the PureSperm technique(P0.01) and the PureSperm combined with swim-up method(P0.01).The titer of sperm-binding antibodies of the PureSperm alone group was significantly lower than that of the PureSperm combination group(P0.05).Conclusions:The sperm quality can be improved by the PureSperm technique alone or combined with swim-up method in those patients with the positive sperm-binding antibodies.The treatment outcome of IUI for patients with immune infertility can be improved when used the sperm samples treated with the PureSperm technique.