Effect of 4-HPR enhancing anti-cancer efficancy of cisplatin to ovarian cancer

OBJECTIVE:To study the effect of 4-HPR combined with DDP on morphological changes,proliferation,apoptosis and cell cycle of human ovarian cancer SKOV3 cells and its relationship with VEGF and u-PA expressions.METHODS:The effect of 4-HPR on morphological changes of SKOV3 cell was observed by an optical microscope.The effect of 4-HPR and DDP on SKOV3 cell proliferation was measured by MTT assay.The cell apoptosis and cell cycle were analyzed by flow cytometry(FCM).The VEGF and u-PA mRNA expressions of SKOV3 cells were detected by RT-PCR analysis.RESULT:One μmol/L of DDP had weak inhibitory effect on SKOV3 cells,more than 5 μmol/L of DDP had remarkable inhibitory effect on SKOV3 cell(P0.05).One μmol/L of 4-HPR had unconspicuous inhibitory effect on SKOV3 cell,and more than 2.5 μmol/L 4-HPR had distinct inhibitory effect on SKOV3 cell(P0.05).Five μmol/L of DDP combined with 4-HPR(1,5 and 10 μmol/L)significantly inhibited the proliferation of ovarian cancer SKOV3 cells,which had a statistical difference compared with the DDP single medication group(P0.05).4-HPR combined with DDP induced cell apoptosis and arrested SKOV3 cell at G2-M and S stage compared with the control and 4-HPR,DDP used alone groups(P0.05).4-HPR combined with DDP decreased the expressions of VEGF and u-PA mRNA compared with the control and 4-HPR,DDP used alone groups(P0.01).CONCLUSION:4-HPR combined with DDP can significantly inhibit the proliferation,induce apoptosis,and block cell cycle of ovarian cancer SKOV3 cells,which is associated with the VEGF and u-PA expressions.