Effects of "Yiqi Huayu Bushen Decoction" serum on articular chondrocytes proliferation and Caspase3/7 activity in rabbits

Objective To observe the effects of Yiqi Huayu Bushen Decoction serum on articular chondrocytes proliferation and Caspase3/7 activity in rabbits.Methods Take the rabbit knee cartilage and separate the chondrocytes nestin.The third generation cells were used to induce cartilage cells degeneration with 10 μg/L speeds of IL-1beta to make Yiqi Huayu Bushen Decoction serum as intervention measure.In observation groups,IL-1beta and different concentrations of Yiqi Huayu Bushen Decoction(5%,10%,20%) were added;and in control group,IL-1beta and different concentrations(5%,10%,20%) of serum-free were added.MTT colorimetric method was adopted to detect chondrocytes proliferation.The third generation of trypanblau growth chondrocytes were then divided into four groups:group A with normal chondrocytes and 20% serum-free,group B with normal chondrocytes and 20% Yiqi Huayu Bushen Decoction serum,group C with IL-1beta-induced chondrocytes and 20% serum-free,group D with IL-1beta-induced chondrocytes and 20% Yiqi Huayu Bushen Decoction serum.In each group,six double holes with 100μl serum were cultured for 24 h and 48 h,and the Caspase3/7 kit was used to assay the Caspase3/7 activity.Results Different concentrations of Yiqi Huayu Bushen Decoction serum could inhibit IL-1beta-induced chondrocytes proliferation.Compared with group A,the caspase3/7 activity was increased in group C(P0.05);compared with group B,the caspase3/7 activity was increased in group D.There was no difference in the Caspase3/7 activity between groups A,B and D(P0.05).ConclusionYiqi Huayu Bushen Decoction can inhibit IL-1beta-induced chondrocytes proliferation by down-regulating Caspase3/7 to suppress chondrocytes apoptosis.