Semi-quantitative Detection of MDR Gene mRNA Expression by Using Multiple RT-PCR

Objective: To establish multiple RT-PCR semi-quantitative detection system to test MDR gene mRNA expression. Methods: Using duplicate RT-PCR as method through a process of amplica tion to become a multi-component RT-PCR, Based on observation of 5 MDR gene's mRNA kinetic effect studies and the setting of RT-PCR standord curves, a six-comples RT-PCR method was established. Results: Six-complex RT-PCR system was identical to simled and duplicated RT-PCR in sensitivity and specificity, and easily to be repeated. Conclusion: This given method was sensitive, specific, and had the advantages of speed in performance (within 6h), only small quantity of specimen (50 mg) and semiquantitation when used to detect the MDR gene mRNA expression in primary liver cancer tissue and cell line. It could also be applied to process a batch of samples simultaneously in one setting.