Effect of Somatostain Analogue on Expression of HIF-1αand VEGF in QBC-939 Cell Line under CoCl_2-induced Hypoxia

Objective To evaluate the effect of somatostain analogue(OCT)on the expression of hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor (VEGF)in QBC-939 cell line under chemical hypoxia induced by CoCl2,and to explore its inhibition on cholangiocarcinoma cell line QBC-939. Methods Cholangiocarcinoma cell line QBC-939 was cultured in vitro under chemical hypoxia induced by CoCl2. The establishment of different concentrations of OCT group(s5,0.5,0.05 and 0.005 mg/L)and control group without OCT was made. Different concentrations of OCT were used to interfere cholangiocarcinoma cell QBC-939. ELISA was used to determine the effects of different OCT doses on the synthesis of VEGF protein in cell culture supernatants of different OCT doses groups. Immunohistochemical technique was used to determine the synthesis of VEGF and HIF-1α protein in QBC-939 cell. Results The levels of VEGF protein in the different concentrations of OCT groups of cell culture supernatants were lower than those of the control group(P0.05). The average grays of HIF-1α and VEGF protein showed a significant difference between any OCT concentration group and the control group(P0.05). HIF-1α was positively correlated with VEGF protein expression in both the different OCT concentrations of groups and the control group(r=0.937, P0.01). Conclusion OCT can inhibit the expression of HIF-1α and VEGF in QBC-939 cell under hypoxia,which may be one of the anti-tumor mechanism,and meanwhile,OCT may inhibit the VEGF expression through inhibition of HIF-1α.