Screening the optimized condition of RAPD - POP

Objective To establish the optimized RAPD - PCR reaction conditions for further genetic monitoring study. Methods Altering the concentrations of the reaction components and the annealing temperature of RAPD - PCR, PCR products from RAPD were electrophoretically separated in agarose gels and banding profiles were visualized by ethidium bromide staining. Recults RAPD - PCR reactions were performed in duplication with SOng of genomic DNA, lSOpmol/L each dNTP, 0. 2xmol/L primer, 1. 5mmol/L magnesium chloride, I.. 5 units of Taq polymerase, annealing at 38t . Under these conditions RAPD method is stable and reproducible. Conclusion The optimized RAPD - PCR reaction conditions were established in this study.