Effect of 11,12-epoxyeicosatrienoic acid preconditioning on the isolated immature rabbit heart

Objective To study the effectiveness of 11,12-epoxyeicosatrienoic acid (11,12-EET) preconditioning in protecting myocardium of immature hearts from ischemic reperfusion injury.Methods On the Langendorff perfusion apparatus,isolated perfused immature rabbit hearts underwent 120 min of normothermic ischemia,followed by 60 min of reperfusion.Twenty-four isolated immature rabbit hearts were divided into three groups randomly: control group,ischemic preconditioning (IPC) group and 11,12-EET preconditioning (EET) group.The preischemia and postischemia myocardial function was assessed by the percentage recovery of left ventricle developed pressure (LVDP),left ventricle end-diastolic pressure (LVEDP),±dp/ptmax,myocardial water content (MWC),and coronary blood effluent (CBE).The index of arrhythmia scores (AS),CK and LDH were also observed.Results The indexes of myocardial function,AS,CK and LDH in IPC and EET groups were significantly better than control group.The values of CK (117.4±8.3) IU/L,LDH (52.7±13.4) IU/L and MWC (75.3±3.5)% in EET group were lower than in IPC group.At the same time,CBE (12.7±1.5) ml was increased significantly.Conclusion 11,12-EET is a superior preconditioning agent to IPC.