Comparison of the frequencies and phenotypes of IL-22-producing T cells from human normal membrane of intestinal tract and peripheral blood mononuclear cells

This paper aimed to compare the characteristics of IL-22-producing T cells in human normal membrane of intestinal tract and peripheral blood mononuclear cells(PBMC).We isolated the mononuclear cells from the membrane of intestinal tract and peripheral blood,respectively.The production of IL-22 by T cells and the correlation of IL-22 with IFN-γ and IL-17 were detected by FACS after stimulation with anti-CD3 plus anti-CD28.Finally,the surface markers CD45RO,CD62L,CCR7,CCR6,CCR10 and CCR4 were analyzed.Compared with the lower secretion of IL-22 by CD4+ and CD8+ T cells from PBMCs(0.6%;0.57%),3.15% of CD4+ IL-22+ and 4% CD8+ IL-22+ could be detected in membrane of intestinal tract after stimulation with anti-CD3 plus anti-CD28.A certain subset of CD4+ IL-22+ and CD8+ IL-22+ T cells was existed and they were distinct from Th1,Th17,Tc1,Tc17 cells.IL-22+ T cells in the membrane of intestinal tract expressed higher levels of CD45RO,part of these cells expressed CCR7 and fewer expressed CD62L.Furthermore,CD4+IL-22+ and CD8+IL-22+ T cells expressed higher levels of CCR10(55.3%;73.9%) and part of CCR6 and CCR4 in the membrane of intestinal tract.In conclusion,IL-22 is mainly produced by the effector or central memory T cells from the membrane of intestinal tract.In addition,a certain subset of IL-22-producing T cells is distinct from Th1,Th17,Tc1,Tc17 cells.