Effect of propofol on expressions of TNF-α,IL-10 and NF-κB in rat cerebrum undergoing ischemia and reperfusion

Objective To study the effects of propofol on the expressions of tumor necrosis factor(TNF)-α,interleukin-10(IL-10)and nuclear factor-κB(NF-κB)in rat cerebrum undergoing ischemia and reperfusion.Methods Thirty rats were randomly divided into five groups with 6 rats each.The rats in group S accepted sharm operation as the controls,in group IR focal cerebral ischemia by temporary bilateral carotid artery clamping,in group PP infusion of propofol 50 mg·kg-1·h-1 at 60 min before ischemia,in group PA infusion of propofol 50 mg·kg-1·h-1 at 10 min after reperfusion,and in group P(without cerebral ischemia)infusion of propofol 50 mg·kg-1·h-1 alone.Concentrations of TNF-α and IL-10 in the cerebrum were measured by enzyme-linked immunosorbance assay(ELISA).An isotope([32P]-ATP)technique was applied for detecting nuclear factor-κB(NF-κB)in rat cerebral cortex.Electrophoretic mobility shift assay(EMSA)was performed in nuclear extracts from cerebral tissues.Results Compared with group S,TNF-α of group IR increased [(2.57±0.19)pg/g vs.(1.60±0.15)pg/g](P0.05),so did the IL-10 [(11.59±1.32)pg/g vs.(7.97±1.96)pg/g](P0.05).Compaered with group IR,TNF-α of group PP decreased [(1.88±0.26)pg/g vs.(2.57±0.19)pg/g](P0.05),so did the IL-10 [(8.35±1.00)pg/g vs.(11.59±1.32)pg/g](P0.05),with decreased activity of NF-κB.There was no significant difference in the levels of TNF-α,IL-10 and NF-κB between group IR and group PA.The changes of IL-10 and NF-κB activity were parallel to that of TNF-α.Conclusion Pre-treatment with propofol can prevent the elevation of inflammatory factors in the cerebrum undergoing ischemia and reperfusion,which was not when used after cerebral ischemia.The effect of propofol on reduction of TNF-α production in ischemic cerebrum may be through inhibiting transduction pathway of NF-κB.