Rapid determination of taxol and its analogues in taxus by solid phase extraction and high performance liquid chromatography

AbstrcatA simple, rapid and sensitive method for the determination of Taxol and its analogues in Taxus using solid phase extraction and high performance liquid chromatography was developed in which a novel high molecular resin was applied. Powdered plant material was extracted with methanol. After being purified by PRP-6 solid phase extraction, the purified extract was analyzed directly by high performance liquid chromatography with a C_(18) column and UV detector at 227 nm. Mixtures of acetonitrile and water (45∶55 in volume ration) were used as mobile phase for elution. The recoveries were between 91% and 98%. Taxol and its analogues in plant extract were separated satisfactorily.