Effect of coixenolide on the proliferation and apoptosis of human HL-60 leukemic cells

OBJECTIVE:To explore the influence of coixenolide on the proliferation and apoptosis of HL-60cells. METHODS:HL-60cells were treated with coixenolide(0,0.5,1,5and 10g/L)for 24,48and 72hours.The cell proliferation was determined by MTT assay,cell apoptosis were measured by AnnexinⅤ/PI staining and apoptosis related protein Caspase-3was determined by Western blot.RESULTS:The proliferation of HL-60cells was inhibited when it was exposed to 0.5and 1g/L coixenolide for 48h,the inhibition rate were(11.510 6±1.165 3)% and(17.095 4±2.695 6)%,respectively.The inhibition was positively correlated to drug concentration and time when the cell line was exposed to 5and 10g/L coixenolide.The different concentrations of coixenolide promoted apoptosis in HL-60cells.Caspase-3was activated and PARP was cleaved when the cell line was exposed to 10g/L coixenolide for 72h.The activated Caspase-3were 17× 103 and 19×103,and the PARP was 89×103.CONCLUSION:Coixenolide can inhibit the growth of HL-60cells through pro-apoptotic action,probably via the activation of Caspase-3.