EFECTS OF EXERCISE TRAINING ON NEURAL FUNCTION AND EXPRESSION OF SEMA3A IN RATS WITH FOCAL CEREBRAL ISCHEMIA

Objective To explore the effects of exercise training on neural function and expression of Sema3A in rats with focal cerebral ischemia(FCI).Methods A total of 30 male SD rats were evenly randomized to three groups as sham-operation group,control group and training group.A model of left middle cerebral artery occlusion for 2 hours was created by intraluminal thread method of rats in the training group;model in the sham-operation group was created by the method as used in the tra-ining group,except blocking the middle cerebral artery.The rats in training group were offered swimming training for 30 min 24 hours after reperfusion for 3 days;those in sham-operation and control groups received no swimming training.MENZIES score,grasp strength and equilibrium function were assessed,the rats were then executed,ischemic side cerebral cortex and hippocampus taken,the expression of Sema3A-mRNA detected by using RT-PCR.Results The score of MENZIES in the rats in training group was lower than that in the control,and higher than that in the training group,the differences being significant(F=177.75;q=19.49,6.00;P0.05);the score of grasp and balance was higher than that in the control,and lower than that in the sham-operation group(F=57.63,117.00;q=7.25-12.46;P0.05).The expression of Sema3A-mRNA in training group was lower than that in the control,but higher than sham-operation group(F=62.873,78.095;q=8.35-9.10;P0.05).Conclusion Exercise training can reduce the expression of Sema3A-mRNA,thus promote the regeneration of axons and improve the function of damaged nerve.