Cloning and Expression of Human IL-18 Gene in E.coli

Objective To highly express IL 18 in E.coli .Methods Extract RNA from human peripheral blood,amplify IL 18 cDNA by RT-PCR,insert into pET 23(b)+vector and transform to E.coli DH5 α.Select positive colonies,extreact recombinant plasmid and transform to E.coli BL21(DE3) for expression.Results The sequence of obtained human IL 18 clones was completely identical to that of IL-18 in GenBank.SDS PAGE proved that the relative molecular weight of the expressed product was about 20000,which was consistent with respected.Conclusion Human IL 18 gene was successfully cloned and expressed in E.coli.