TOXICITIES OF BISPHENOL A ON DIFFERENT DEVELOPED STAGES OF ZEBRAFISH(DANIO RERIO)AND THEIR MECHANISMS

To evaluate the toxic effects of bisphenol A(BPA)on aquatic organism,different developed stages of zebrafish(embryo,larva and adult fish) were exposed to BPA solution,and the DNA break determination was done on zebrafish embryo.The toxic mechanisms of BPA on zebrafish were discussed primarily.Results showed the 24h LC50 of BPA on adult fish(8.00—10.00mg·l-1) was significant lower than that on the embryo(16.4±0.40mg·l-1),indicated adult zebrafish was more suitable for acute experiment while embryos were significant for the studies of sub-acute and genetic toxicity.While exposed to 5.00 mg·l-1 BPA,the BPA contents in embryo and adult fish were 373.57±39.76μg·g-1 and 155.78±17.97μg·g-1,which suggested that bio-accumulated ability to BPA in embryo was nearly twice of that in adult fish.This study also proved a result of DNA break in zebrafish embryo exposed to a lower BPA solution(5.00mg·l-1).