2007•Food ScienceRequires access
Experiment Study on Utilization of Chicken Liver Esterase in Determination of Organophosphate and Carbamate Pesticides
Fei Xu
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Abstract
Based on the esterase inhibition actions of pesticides,different concentrations of dichlorvos,trichlorfon,malathion and carbaryl were determined by free and immobilized chicken liver esterase.The results showed that there are reasonable linear relationships between the logarithm of pesticides'concentrations and the inhibition ratio of esterase activity in lower concentration zones both for free and immobilized enzymes.With application of immobilized chicken liver esterase,the detection limits are 0.001,4.34 and 2.52mg/L respectively for dichlorvos,malation and carbaryl while after the free chicken liver esterase immobilized treatment,they are respectively lowered to 0.00004,0.0004 and0.78mg/L.However there is no obvious improvement in determination limit for trichlorfon with immobilized esterase.