The expression of keratin 16 in the experimental amniotic fluid embolism

Objective To study the significance of keratin 16 in forensic,replicate the animal models of amniotic fluid embolism after injecting the different nature and doses amniotic fluid.Methods The Wistar female rats were randomly divided into 3 groups,there was the amniotic liquid group(AF),the mixture of amniotic fluid meconium group(MAF) and the control group(NS),and different doses of amniotic fluid were injected.The expression of keratin 16 was observed by the HE staining and immunohistochemical staining after the rats dying.Results The expression of cytokeratin 16 was negative in the control group,was positive in AF group and MAF group,and the numbers of keratin 16 expression increased when increasing amniotic fluid.Conclusions The expression of cytokeratin 16 relevants with the amniotic fluid into the blood,not relevant with the level of meconium-stained amniotic fluid.