Study on Tissue Culture and Rapid Propagation of Gazania rigens L.

In vitro seedling leaves of Cazania leaves were used as explants,by using MS as minimal medium,adding different concentration of TDZ,2,4-D,IAA,IBA,effect of different concentration hormone and their combination on the induction of adventitious buds,rooting and transplanting,practice of test-tube seedling were studied.The results indicated that optimum medium for induction adventitious buds was MS+TDZ 0.8 mg/L+IBA(0.1～0.5)mg/L,induction rate of more than 90.0%.The best rooting medium was 1/2MS+IBA 0.5 mg/L+NAA 0.2 mg/L,the rooting rate reached 100%.The leaf explants of Cazania was a better choice,in the short term to obtain large amounts of tissue culture seedlings,the transplanting survival rate could reach more than 95%.