Biodistribution pharmacokinetics and toxic effect of P-32 chromic phosphate colloids interstitially injected into human pancreatic cancer (Pc-3)-bearing nude mice

Objective To study the biodistribution,pharmacokinetics and toxic effect of chromic phosphate colloids (P-32 colloids) interstitially administered into BALB/c-nu/nu nude mice bearing Pc-3 human pancreatic carcinoma. Methods Fifty-one nude mice were injected with various doses of P-32 colloids through caudal vein or into the tumor by interstitial injection.The radioactive distribution of P-32 colloids in mouse bodies was observed dynamically and white blood cells and platelets were counted,body weight and morphology were studied,and the tumor surface radioactive counts were also measured. Results After intratumoral administration of P-32 colloids,the radioactive counts per minute in the tumor were obviously higher than those in other tissues or organs,and the radioactive counts per minute of other tissues or organs were evidently lower than those after caudal vein administration.The effective half life of P-32 colloids injected was 13 days.Morphological examination demonstrated that most of Pc-3 cells were destroyed after intratumoral administration of P-32 colloids,and differentiated benign tumor cells were detected.The radiation lesions of liver,spleen,lung,lymph node and other important tissues or organs were reversible.No obvious bone marrow depression was detected. Conclusion It is demonstrated that the intratumoral injection of P-32 colloids is a safe,easy and effective radionuclide interventional therapy for pancreatic tumor. ;