Quantitative Analysis of Narigin and Hesperidin contents in Citrus Aurantium Juice by Reverse-phase High-performance Liquid Chromatography

The chromatographic conditions for quantitative analysis of narigin and hesperindin contents in citrus aurantium juice were investigated and the optimal reverse-phase chromatogram conditions were found as follows: methanol/water being of 40/60, column being of ODS-C18 (4.6μm×150mm), flow rate of 1mL/min and the detection wavelength being of 285nm. It was also found that the contents of narigin and hesperidin in citrus aurantium juice via methanol dissolve and supersonic treatment were 32% and 4.4% higher than those via water dissolve and standing storage, respectively. The method was simple and accurate.