Effects of Homocysteine on Function of Human Peripheral Blood Progenitor Cells

【Objective】 This study investigated the effect of moderate concentration of homocysteine(Hcy) on the migration and adhesion function of human endothelial progenitor cells(EPC). 【Method】 Total mononuclear cells were isolated from human peripheral blood by Ficoll density gradient centrifugation. The cells were plated on fibronectin-coated dishes, and then cultured in EGM-2 medium. After 7 days culture, the attached cells were identified by immunofluorescence. EPC were divided into control group(CT), 50 μmol / L adenosine group(A50), 50 μmol / L Hcy group(H50), 50 μmol / L Hcy+50 μmol / L Ade group(A50+H50) and50 μmol / L Hcy + 50 μmol / L Ade + 10 ng / mL VEGF group(A50+H50+VEGF). EPC migration and adhesion were detected. All data were from at least three independent experiments. 【Result】 EPC were characterized as double positive for Dil-acLDL and FITClectin. The migration and adhesion of EPC were significantly attenuated in H50 and A50 group compared with the CT group(P 0.05). Hcy combined with Ade further inhibited EPC migration and adhesion compared with H50 and A50 group(P 0.05). The supplement of recombinant VEGF promoted EPC migration and adhesion(P 0.05). 【Conclusion】 Hcy can significantly inhibit EPC migration and adhesion, which was abolished by the supplement of recombinant VEGF.