Study on the cytopathic effect properties of newscastle disease virus adapted to grow in different cell lines

Virulent and attenuated newscastle disease virus(NDV) were adapted to grow in the DF-1,BHK-21 and HeLa cell lines in order to investigate different cytopathic effect(CPE) in various cell lines.The results showed that virulent NDV could produce typical cytopathic effect after serial passages in various cell lines,as indicated by CPE of rounding,degeneration and the formation of synctia in virous cell line.But the HA titer and proliferation rate were better in DF-1 cell line than those in BHK-21 and HeLa cell lines.When the concentration of trypsin was 10μg/mL in DMEM,attenuated NDV was confirmed circular and desquamated without typical CPE,no modification in the CPE properties was detected after fifteen passages,also the HA titer displayed zero.It appears that NDV strain grew in DF-1 culture has retained its biological homology,so the use of DF-1 cell line could be the best cell culture for the infection of NDV.