2006•Shiyong yixue zazhiRequires access
Effects of erythropoietin on arrhythmia induced by myocardial ischemia-reperfusion in rats
Yanqing Wang
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Abstract
Objective To observe the effects of erythropoietin on arrhythmia induced by myocardial ischemia-reperfusion in rats.Methods The rat model of myocardial ischemia-reperfusion injury(IRI) was set up by ligating of left anterior descending coronary artery for 30 minutes and then loosing for 120 minutes. Sixty SD rats were randomly divided into three groups: sham-operation group(n=20),ischemia-reperfusion group(n=20) and EPO group(n=20). During the experimental procedures,lead Ⅱ ECG was traced continuously for the arrhythmia induced by ischemia or reperfusion. The levels of myocardial malonaldehyole (MDA),superoxide dismutase (SOD),glutathione peroxidase(GSH-Px),catalase (CAT),Na+ -K+ -ATPase and Ca2+ -ATPase were detected at 120 min after reperfusion. Results Erythropoietin decreased the incidence and the duration of ventricle arrhythmia induced by reperfusion,resulting in the scores of arrhythmia decrease significantly. Erythropoietin reduced myocardial MDA content,enhanced the activity of myocardial GSH-Px,CAT,Na+ -K+ -ATPase,Ca2+ -ATPase at 120 min after reperfusion but didn′ t affect the activity of myocardial SOD. Conclusion Erythropoietin may effectively prevent ventricle arrhythmia induced by myocardial ischemia-reperfusion,and its mechanism may be related to the decrease of the injury caused by oxygen free radical and calcium overload.