Anti-proliferative Effects and Mechanisms of Serum Containing Simiao Yongan Decoction on Rabbit Aortic Smooth Muscle Cells During the Insulin Resistance State

Objective:To observe the anti-proliferative effects and possible mechanisms of serum containing Simiao Yongan decoction(SYD) on rabbit aortic smooth muscle cells(VSMC) during the insulin resistance state.Method:Nine New Zealand big-eared rabbits were randomly divided into 3 groups with 3 rabbits each group.Each groups were separately given distilled water 10 mL,simvastatin 5 mg·kg-1 and SYD at dosage of 11.25 g·kg-1 each time and twice a day for 3 consecutive days.Serum containing SYD was prepared by serum pharmacological method,the proliferation of VSMC was induced by high concentrations of glucose and insulin(4 500 mg·L-1 glucose + 100 U·L-1insulin).The VSMCs were divided into following groups:blank group(10%FBS normal glucose DMEM),normal group(10%FBS normal glucose DMEM + normal serum containing),model group(10% FBS glucose DMEM + 100 U·L-1 insulin + normal serum containing),western medicine group(10%FBS high glucose DMEM +100 U·L-1 insulin + serum containing simvastatin),traditional Chinese medicine group(10% FBS high glucose DMEM + 100 U·L-1 insulin + serum containing SYD).Cell proliferation was measured by methyl thiazolyl tetrazolium(MTT) assay,content of IL-6 and TNF-α in cell culture supernatant was detected by enzyme linked immunosorbent assay(ELISA) method.Result:The proliferation of VSMC was markedly induced by high concentrations of glucose and insulin.Compared with the normal group,the absorbance(A) value of MTT,the content of cell culture supernatant of IL-6 and TNF-α in model group was increased significantly(P 0.05);compared with the model group,Chinese medicine group and western medicine group,the A value,the content of IL-6 and TNF-α of cell culture supernatant was reduced(P 0.05).Conclusion:Serum containing SYD can inhibit proliferation of the smooth muscle cells induced by high glucose and insulin,one of the possible mechanisms may be related to reducing secretion of IL-6 and TNF-α in smooth muscle cells.