Effects of Arbuscular Mycorrhiza Fungi on Seedlings Growth and Antioxidant Systems of Leaves in Cucumber Under Low Temperature Stress

【Objective】The aim of this experiment was to study the effects of arbuscular mycorrhiz fungi(AMF) on the growth,antioxidant enzyme activities and other physiological indices in cucumber(Cucumis sativus L.) seedlings.【Method】Cucumber cultivar ‘Jinchun 2’ was subjected to low temperature treatment of 15/10℃(day / night) in the artificial climate chamber.The effects of AMF on the growth and the changes in electrolyte leakage rate,root activity,chlorophyll,soluble protein and malondialdehyde(MDA) contents,superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) activities under low temperature stress in the seedling leaves were investigated.【Result】Under low temperature stress,the increments of height,stem diameter,fresh weight and dry weight of root or shoot were suppressed.Compared with the control treatment,AMF-inoculation treatment could significantly promote the increment of height,stem diameter,fresh weight,dry weight,chlorophyll contents by 59.9%-81.7%,soluble protein content by 10.24%,root activity by 178.32%,and increased SOD,POD,CAT activities by 57.63%,6.72% and 35%,while reduced MDA contents and electrolyte leakage by 11.05% and 16.08% in cucumber seedlings leaves under low temperature stress.【Conclusion】 AMF-inoculation could promote the accumulation of proline,raise the activities of antioxidant enzymes,decrease the membrane lipid peroxidation and improve the adaptability of cucumber seedlings under low temperature stress.