ScholarLens Back to search
2009•Huazhong Nongye Daxue xuebaoRequires access

Identification of Candidate Single Nucleotide Polymorphisms in Poplar Based on Expressed Sequence Tags

Xinye Zhang

Open publisher page 0 citations

Abstract

More than 20 000 EST sequences of poplar were clustered and stringent filters were used to identify reliable candidate SNPs.Sequences analysis indicated that totally 94 candidate SNPs were found.The main types of substitutions among candidate SNPs were A/G,C/T and other types,which accounted for 32.98%,13.83%,and 53.20%,respectively.Resequencing with 6 samples for 20 candidate SNPs showed that 13 candidate SNPs were verified,accounting for 65%.And among them,11 SNPs were substitution type and the other 2 SNPs were heterozygous type.The detailed information about these candidate SNPs would provide a basis for further marker identification and application.

About this research paper

What this paper is about

More than 20 000 EST sequences of poplar were clustered and stringent filters were used to identify reliable candidate SNPs.Sequences analysis indicated that totally 94 candidate SNPs were found.The main types of substitutions among candidate SNPs were A/G,C/T and other types,which accounted for 32.98%,13.83%,and 53.20%,respectively.Resequencing with 6 samples for 20 candidate SNPs showed that 13 candidate SNPs were verified,accounting for 65%.And among them,11 SNPs were substitution type and the other 2 SNPs were heterozygous type.The detailed information about these candidate SNPs would provide a basis for further marker identification and application.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

More than 20 000 EST sequences of poplar were clustered and stringent filters were used to identify reliable candidate SNPs.Sequences analysis indicated that totally 94 candidate SNPs were found.The main types of substitutions among candidate SNPs were A/G,C/T and other types,which accounted for 32.98%,13.83%,and 53.20%,respectively.Resequencing with 6 samples for 20 candidate SNPs showed that 13 candidate SNPs were verified,accounting for 65%.And among them,11 SNPs were substitution type and the other 2 SNPs were heterozygous type.The detailed information about these candidate SNPs would provide a basis for further marker identification and application.

Key concepts: Single-nucleotide polymorphism, Candidate gene, Genetics, Biology, SNP genotyping, Computational biology, Gene, Genotype

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Identification of Candidate Single Nucleotide Polymorphisms in Poplar Based on Expressed Sequence Tags — Research Paper | ScholarLens