Effect of NG-nitro-L-Arginine and L-Arginine on mitochondria injury caused by acute lung injury induced by LPS in rats

Objective To observe the effect of NG-Nitro-L-Arginine (L-NA)and L-Arginine(L-Arg)on mitochondria injury caused by acute lung injury induced by LPS in rats.Methods The rats were randomly devided into blank control group,LPS injury group,L-NA group,L-NA+L-Arg group and L-Arg group.The models of acute lung injury were prepared with injection of LPS in rat.L-NA and L-Arg were respectively administrated through intraperitioneal injection at 3h after injury induced by LPS,and the rats were killed and the mitochondria of lungs was isolated by differential centrifugation.The activities of T-NOS,iNOS,ATPase,SOD and GSH-Px and the contents of NO and MDA from mitochondria were respectively detected.Results The activities of T-NOS and iNOS were significantly increased,however,the activities of ATPase,SOD and GSH-Px were significantly decreased,and the contents of NO and MDA were increased after acute lung injury.The L-NA and L-Arg could significantly increase ATPase,SOD and GSH-Px activities,and could decrease NO and MDA contents and iNOS activity in mitochondria injury from acute lung injury induced by LPS in rats.Conclusion The L-NA and L-Arg can effectively inhbit the activity of iNOS,enhance the activity of eNOS,improve mitochondria energy pump,change the production of NO,ameliorate oxidative injury,and effectively protect lung tissue against acute lung injury induced by LPS.