Investigation of Soil Anaerobic Sporeforing Bacilli in Tibet High Altitude

Objective To investigate the situation of soil anaerobic sporeforing bacilli in Tibet high altitude including its class, number and the sensibility to drugs, which may help diagnosis and treatment of infection in peace and war time. Methods According to Bergey's manual, bacteria from soil sample were cultured in anoxic condition, isolated and differentiated by biochemistry reaction. Results The number of anaerobic sporeforing bacilli in per gram soil was 30 and 162 000. The number of all kinds of bacteria was 0.11×10 7～88.6×10 7 per gram. There were great differences in bacterial numbers of different points and different depths soil in the same area. There was no anaerobic sporeforing bacilli be found in unsorted land and soil deeper than 75 cm. In all isolated 27 classes, 96 strains of bacterium, Clotridium per fringens accounts for 20.4 percent. Conclusion There were anaerobic sporeforing bacilli and other anaerobes responsible for gas gangrene in Tibet soil, which mainly come from feces of mankind and animals. The sensitive rates to common antibiotics fo these bacteria were all above 90 percent.