Levels of VEGF,IL-6 and Leptin in the aqueous humor of type 2 diabetic patients

Objective To explore the contents and clinical significant of vascular endothelial growth factor VEGF,interleukin-6 IL-6,Leptin in the aqueous humor of type 2 diabetic retinopathy.Methods According to eyeground examination and fundus fluorescein angiography,70 2-diabetic patients 70 eyes were divided into two groups,experimental group which subdivided into diabetic patients without retinopathy NDR,22 cases 22 eyes,background diabetic retinopathy BDR,28 cases 28 eyes,proliferative diabetic retinopathy PDR,20 cases 20 eyes,and control group with normal senile cataract patients 20 cases 20 eyes.The contents of VEGF and IL-6 in the aqueous humor were determined by using the ELISA technique and the content of Leptin was measured with radioimmunoassay.Results The level of VEGF in the aqueous humor samples of NDR,BDR and PDR were 250.3226.77ng·L-1,300.1158.89ng·L-1 and 496.2391.06ng·L-1 respectively.The level of IL-6 in the aqueous humor of NDR,BDR and PDR were 162.8133.92ng·L-1,256.7664.15ng·L-1 and 391.2790.46ng·L-1 respectively.The level of Leptin in the aqueous humor of NDR,BDR and PDR were 69.8021.37ng·L-1,155.0832.76ng·L-1 and 230.2756.92ng·L-1 respectively.The levels of VEGF,IL-6,Leptin in the aqueous humor of the control group were 144.6926.55ng·L-1,86.7122.69ng·L-1 and 43.6220.02ng·L-1 respectively.Compared with the control,the levels of VEGF,IL-6 and Leptin in the aqueous humor of experimental groups were different significantly F=118.62,P0.01;F=110.53,P0.01;F=101.22,P0.01.The levels of VEGF,IL-6 and Leptin in the aqueous of the NDR,BDR and PDR increased significantly.The level of VEGF significantly correlated with the level of IL-6 and Leptin in the aqueous humorr=0.995,P=0.000;r=0.776,P=0.000.The level of VEGF,IL-6 and Leptin in the aqueous humor also significantly correlated with the severity and prolongation of DRr=0.722,P=0.000;r=0.716,P=0.000,r=0.869,P=0.000;r=0.865,P=0.000,r=0.776,P=0.000;r=0.765,P=0.000.Conclusion VEGF and IL-6 play active roles in the generation and development of diabetic retinopathy,and the level of VEGF in the aqueous humor closely correlates with the level of IL-6 and Leptin in the aqueous humor of diabetic patients.