2003•Chongqing Yike Daxue xuebaoRequires access
Effect of c-erbB2 antisense oligodexynucleotides on radiosensitivity of human ovarian cancer cell line
Ren Qinglan
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Abstract
Objective:To explore the effect of lipofectin-c-erbB2 antisense oligodexynucleotides on radiosensitivity of human ovarian cancer cell line.Methods:The expression of c-erbB2 was detected by means of RT-PCR;cellular response to irradiation was evaluated by MTT test and the colony forming assay.Results:Lipofectin-c-erbB2 antisense oligodexynucleotides(AS-ODN) could suppress the expression of c-erbB2,and significantly decreased the survival fraction and colony forming rate of human ovarian cancer cells after ionizing irradiation( P 0.01), while non-treament and the sense oligodexynucleotides(S-ODN) groups did not decrease on the radio-resistance level of SKOV3 cell line( P 0.05).Conclusion:c-erbB2 antisense oligodexynucleotides sensitize the SKOV3 to ionizing irradiation through decreasing the expression of c-erbB2,which might be the result of the fact that c-erbB2 antisense oligodexynucleotides inhibit the cellular signal transduction pathway relating to the radiation-resistant phenotype.