2012•Qilu yixue zazhiRequires access
THE INFLUENCE OF L-CARNITINE ON EXPRESSIONS OF Nrf2 AND γ-GCS IN RENAL ISCHEMICAL REPERFUSION INJURY
Jian Gao
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Abstract
Objective To investigate the influence of L-carnitine on expressions of Nrf2 and γ-GCS in renal ischemical reperfusion injury(RIRI).Methods Experimental rats were randomized to sham-operation group,ischemia-reperfusion(IR) group and treatment group.A model of RIRI in rats was created in IR group and treatment group.For the rats in treatment group,2 mL of L-carnitine was injected into a caudal vein before establishment of the model.No IR procedure was done for the rats in sham-operation group.The experimental rats were sacrificed six hours after IR,serum Cr,BUN,Cys C were then detected,and the activity of serum SOD and content of MDA in serum were also measured.The expressions of Nrf2 and γ-GCS in renal tissue were measured by using RT-PCR and Western-blot.Results The levels of Cr,BUN,Cys C and MDA in the treatment group were lower than that in IR group,and the activity of SOD was higher(F=8.58-57.42,q=4.06-11.26,P0.05).Compared with the sham-operation group,the expressions of Nrf2,γ-GCS mRNA and protein in renal tissue of IR group were higher,and the parameters of above items in the treatment group were even higher than that in IR group(F=143.53-241.64,q=3.76-13.51,P0.05).Conclusion L-carnitine can relieve ischemical reperfusion injury in kidney,which realizes this target is likely by way of activating Nrf2-ARE and inducing the expression of γ-GCS.