Protective Effects of Sodium Ferulate on Human Proximal Tubular Epithelial Cells Damage Induced by High Glucose

Objective To observe the influence of sodium ferulate(SF)on proliferation and apoptosis of human proximal tubular epithelial cells(HKCs)induced by high glucose.Methods The renal tubular epithelial cells in logarithmic growth phase were divided into control group,high glucose group and high glucose plus SF group.The cell proliferation ability was measured by MTT assay.The culture solution concentrations of lactate dehydrogenase(LDH)were measured to evaluate cell injury.The culture solution of malondiadehycle(MDA)levels and superoxidedismutase(SOD) activities were measured to assess oxidative stress,and cell apoptosis were detected with Hoechst 33258.Results MTT absorbance(OD value)of high glucose group was decreased significantly along with the culture time compared with control group.The OD value of high glucose plus SF group was reduced significantly compared with high glucose group(P0.05).With the prolongation of culture time,the culture solution concentration of LDH was increased significantly and were(17.55± 2.45)and(28.72±3.11)U/L at 12 and 24 hours respectively.LDH levels were reduced to(11.84±2.18)and(19.98± 3.67)U/L at 12 and24 hours in high glucose plus SF group(P0.05).The culture solution of MDA levels were higher(P 0.01),the SOD activities were significantly reduced(P0.01)in high glucose group than that in control group.The MDA levels were significantly reduced(P0.01)and SOD activities were significantly increased(P0.05 or 0.01)in high glucose plus SF group.Hoechst 33258 fluorescent staining revealed that the nucleus crimpled,crescent liked and chromatin condensed,even disintegrated.The apoptosis rate of high glucose group(34.17%) was higher than that in the control group(5.20%)and high glucose plus SF group(7.39%,P 0.01).Conclusion High glucose can induce HKC injury and apoptosis,and inhibit its proliferation.The protective of SF may be correlated with its effects to inhibit damage and apoptosis,and improve the proliferation ability of HKC.