Multi-enzyme PFGE Scheme for Subtyping Campylobacter Jejuni
Jinling Shen
Abstract
Jinling Shen
Abstract
To enhanced discriminatory scheme for subtyping and traiting back Campylobacter jejuni,6 enzymes were used in 33 Campylobacter jejuni PFGE typing.BamHⅠ is the best restriction enzyme for subtyping Campylobacter jejuni,A dendrogram derived from the combined analysis of BamHⅠ and additional 2 restriction enzymes KpnⅠ and SacⅡ was highly discriminatory.
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To enhanced discriminatory scheme for subtyping and traiting back Campylobacter jejuni,6 enzymes were used in 33 Campylobacter jejuni PFGE typing.BamHⅠ is the best restriction enzyme for subtyping Campylobacter jejuni,A dendrogram derived from the combined analysis of BamHⅠ and additional 2 restriction enzymes KpnⅠ and SacⅡ was highly discriminatory.
Key concepts: Subtyping, Campylobacter jejuni, Pulsed-field gel electrophoresis, Campylobacter, Campylobacter coli, Microbiology, Typing, Restriction enzyme