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2011•Progress in Veterinary MedicineRequires access

Determination of Virulence of SD Strain of Infectious Bursal Disease Virus

WU Yi-chun

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Abstract

An infectious bursal disease virus field strain,designated as strain SD,was isolated from a flock with immune failure.Using serological method,embryo inoculation test,HA,electron microscopic observation,the strain was identified as a pure IBDV isolate.The viral titer(ELD50) reached 10-6.5/0.2 mL;Four-week-old chickens inoculated with the strain developed severe clinical disease with a morbidity of 100%,and a mortality of 85%.About pathological examination,the most characteristic gross lesions were severe bursal haemorrhage and thymic atrophy,as well as the bone marrow with fatty change.So the SD strain was confirmed as a very virulent strain of IBDV.

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What this paper is about

An infectious bursal disease virus field strain,designated as strain SD,was isolated from a flock with immune failure.Using serological method,embryo inoculation test,HA,electron microscopic observation,the strain was identified as a pure IBDV isolate.The viral titer(ELD50) reached 10-6.5/0.2 mL;Four-week-old chickens inoculated with the strain developed severe clinical disease with a morbidity of 100%,and a mortality of 85%.About pathological examination,the most characteristic gross lesions were severe bursal haemorrhage and thymic atrophy,as well as the bone marrow with fatty change.So the SD strain was confirmed as a very virulent strain of IBDV.

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Available abstract

An infectious bursal disease virus field strain,designated as strain SD,was isolated from a flock with immune failure.Using serological method,embryo inoculation test,HA,electron microscopic observation,the strain was identified as a pure IBDV isolate.The viral titer(ELD50) reached 10-6.5/0.2 mL;Four-week-old chickens inoculated with the strain developed severe clinical disease with a morbidity of 100%,and a mortality of 85%.About pathological examination,the most characteristic gross lesions were severe bursal haemorrhage and thymic atrophy,as well as the bone marrow with fatty change.So the SD strain was confirmed as a very virulent strain of IBDV.

Key concepts: Infectious bursal disease, Virulence, Virology, Virus, Strain (injury), Serology, Titer, Biology

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