Cytogenetical analysis on 1169 couples with adverse pregnancy history

Objective:To analyze chromosomal karyotypes of the couples with adverse pregnancy history,explore the relationship between adverse pregnancy outcome and chromosomal abnormality,provide genetic consultation for secondary pregnancy. Methods:The venous blood samples of 1 169 couples with adverse pregnancy history were obtained,then the lymphocytes were cultured,metaphase was gained,after hypotonicity,fixation,preparation of sections and Giemsa staining,karyotype analysis under microscope was carried out. Results:Among 2 338 cases(1 169 couples),95 cases were found with abnormal karyotype,including 50 women and 45 men,the abnormal rate was 4.06%.3 cases were found with numerical abnormality of chromosomes,the abnormal rate was 0.13%.92 cases were found with structural abnormality of chromosomes,the abnormal rate was 3.93%,including 34 cases with balanced translocation,10 cases with Robertsonian translocation,27 cases with inversion,2 cases with partial repetition,18 cases with mutation of chromosomal polymorphism and 1 case with dicentric chromosome,there was no significant difference in the abnormal rate of chromosomal karyotype between the cases with different times of abortion and different genders. Conclusion:Chromosomal abnormality is an important reason of adverse pregnancy outcome,there is no significant difference in the rate of chromosomal abnormality between the cases with different times of abortion and different genders,the couples with adverse pregnancy outcome should both receive cytogenetical examination,and the couples with abnormal chromosomal karyotype should receive genetic consultation and prenatal diagnosis to reduce the birth rate of infants with chromosomal abnormality when the couples get secondary pregnancy.