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2004•Zhongguo yufang shouyi xuebaoRequires access

Cloning and sequence analysis of the portional gene of FPV

Hong Zhang

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Abstract

According to the reported complete nucleotide sequence of FPV in GenBank,a pair of primers were designed and synthesized.By the method of PCR,the NS1 gene of isolate was amplified.The products of PCR were cloned into pMD18_T vector and sequenced.It revealed that the full length of NS1 gene was 624?nt and encoded 204 amino acids.Compared the NS1gene of isolate with CPV、MEV,it showed that they shared 99?%、99?% identity innucleotide sequence and 99?%、99?% identity in deduced amino acid sequence respectively.

About this research paper

What this paper is about

According to the reported complete nucleotide sequence of FPV in GenBank,a pair of primers were designed and synthesized.By the method of PCR,the NS1 gene of isolate was amplified.The products of PCR were cloned into pMD18_T vector and sequenced.It revealed that the full length of NS1 gene was 624?nt and encoded 204 amino acids.Compared the NS1gene of isolate with CPV、MEV,it showed that they shared 99?%、99?% identity innucleotide sequence and 99?%、99?% identity in deduced amino acid sequence respectively.

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Available abstract

According to the reported complete nucleotide sequence of FPV in GenBank,a pair of primers were designed and synthesized.By the method of PCR,the NS1 gene of isolate was amplified.The products of PCR were cloned into pMD18_T vector and sequenced.It revealed that the full length of NS1 gene was 624?nt and encoded 204 amino acids.Compared the NS1gene of isolate with CPV、MEV,it showed that they shared 99?%、99?% identity innucleotide sequence and 99?%、99?% identity in deduced amino acid sequence respectively.

Key concepts: GenBank, Biology, Cloning (programming), Gene, Nucleic acid sequence, Genetics, Sequence analysis, Sequence (biology)

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